Tuesday, August 17, 2010

If you used E coli DNA polymerase instead of Thermus aquaticus DNAA ploy. in PCR proced. what would be result?

This would be in the PCR procedure. I need to know would the result be: PCR would be twice as fast, would PCR stop after 1 cycle, PCR would occur more slowly that normal, PCR not occur at all, or Many mistakes would be made??? Please help, I am trying to do research for a clinical and this is the only thing I am stuck on.

If you used E coli DNA polymerase instead of Thermus aquaticus DNAA ploy. in PCR proced. what would be result?
PCR would not occur at all, during the stage where the DNA dissociates, the E.coli DNA polymerase would denature and therefore would not be able catalyze the creation of DNA.


No comments:

Post a Comment